Proteins (Methods in Molecular Biology, 1)

Synopsis

The Lowry Method For Protein Quantitation / Jaap H. Waterborg, Harry R. Matthews -- Determination Of Protein Molecular Weights By Gel Permeation High Pressure Liquid Chromatography / E.l. Mayes -- Immunoaffinity Purification Of Protein Antigens / E.l. Mayes -- Electrophoretic And Chromatographic Separation Of Peptides On Paper / E.l. Mayes -- Peptide Mapping By Reverse-phase High Pressure Liquid Chromatography / E.l. Mayes -- Sds Polyacrylamide Gel Electrophoresis Of Proteins / B.j. Smith -- Gradient Sds Polyacrylamide Gel Electrophoresis / John M. Walker -- Acetic Acid-urea Polyacrylamide Gel Electrophoresis Of Proteins / B.j. Smith -- The In Vivo Isotopic Labeling Of Proteins For Polyacrylamide Gel Electrophoresis / Jeffrey W. Pollard -- Two-dimensional Polyacrylamide Gel Electrophoresis Of Proteins / Jeffrey W. Pollard -- Starch Gel Electrophoresis Of Proteins / Graham B. Divall -- Isoelectric Focusing In Ultrathin Polyacrylamide Gels / Graham B. Divall --^ High-sensitivity Silver Staining Of Proteins Following Polyacrylamide Gel Electrophoresis / Keith Gooderham -- Quantification Of Proteins On Polyacrylamide Gels (nonradioactive) / B.j. Smith -- Computer Analysis Of Gel Scans / Harry R. Matthews -- Drying Gels / Bryan J. Smith -- Fluorography Of Polyacrylamide Gels Containing Tritium / Jaap H. Waterborg, Harry R. Matthews -- Recovery Of Proteins From Dried Polyacrylamide Gels After Fluorography / Jaap H. Waterborg, Harry R. Matthews -- The Electrophoretic Elution Of Proteins From Polyacrylamide Gels / Jaap H. Waterborg, Harry R. Matthews -- Transfer Techniques In Protein Blotting / Keith Gooderham -- Peptide Mapping By Thin-layer Chromatography And High Voltage Electrophoresis / Keith Gooderham -- In Situ Peptide Mapping Of Proteins Following Polyacrylamide Gel Electrophoresis / Keith Gooderham -- The Dansyl Method For Identifying N-terminal Amino Acids / John M. Walker --^ The Dansyl-edman Method For Peptide Sequencing / John M. Walker -- Microsequencing Of Peptides And Proteins With 4-n, N-dimethylaminoazobenzene-4'-isothiocyanate / Brigitte Wittmann-liebold, Makoto Kimura -- Manual Edman Degradation Of Proteins And Peptides / Per Klemm -- Carboxy-terminal Sequence Determination Of Proteins And Peptides With Carboxypeptidase Y / Per Klemm -- Immunization And Fusion Protocols For Hybridoma Production / J.n. Wood -- Immunofluorescence And Immunoperoxidase Screening Of Hybridomas / J.n. Wood -- Solid-phase Screening Of Monoclonal Antibodies / J.n. Wood -- Subclass Analysis And Purification Of Monoclonal Antibodies / J.n. Wood -- The Production Of Antisera / G.s. Bailey -- Immunodiffusion In Gels / G.s. Bailey -- Crossed Immunoelectrophoresis / Graham B. Divall -- Rocket Immunoelectrophoresis / John M. Walker -- Radioiodination Of Proteins / G.s. Bailey -- Radioimmunoassay / G.s. Bailey -- Enzyme-linked Immunosorbant Assay (elisa) / Wim Gaastra. Edited By John M. Walker. Includes Bibliographical References And Index.